Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE087 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500 pg/ml	2.991	3.136	3.031
	3.281
250 pg/ml	1.613	1.631	1.526
	1.649
125 pg/ml	0.971	0.925	0.82
	0.879
62.5 pg/ml	0.532	0.512	0.407
	0.493
31.25 pg/ml	0.298	0.307	0.202
	0.316
15.625 pg/ml	0.202	0.207	0.102
	0.213
7.8125 pg/ml	0.158	0.155	0.05
	0.153
0 pg/ml	0.104	0.105	0
	0.106

We measured random samples of BPE087 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	48.3	1.6	3.4
Intra-assay	2	20	142	4.5	3.2
Intra-assay	3	20	365	15.2	4.2
Inter-assay	1	20	48.5	6.5	13.4
Inter-assay	2	20	142	16.9	11.9
Inter-assay	3	20	362	38.9	10.7

> Protocol Booklet

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