Full product name

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Sample type

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Assay time

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Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE361 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500 pg/ml	2.735	2.738	2.606
	2.741
250 pg/ml	1.575	1.636	1.504
	1.697
125 pg/ml	0.903	0.934	0.802
	0.965
62.5 pg/ml	0.521	0.529	0.397
	0.537
31.25 pg/ml	0.34	0.337	0.205
	0.334
15.625 pg/ml	0.227	0.23	0.098
	0.233
7.8125 pg/ml	0.171	0.18	0.048
	0.188
0 pg/ml	0.128	0.132	0
	0.136

We measured random samples of BPE361 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	41.2	1.1	2.8
Intra-assay	2	20	82.4	2.4	2.9
Intra-assay	3	20	358	11.1	3.1
Inter-assay	1	20	41.3	2.8	6.7
Inter-assay	2	20	83.3	5	6
Inter-assay	3	20	354	27.8	7.8

> Protocol Booklet

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