Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE366 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500 pg/ml	2.989	2.89	2.796
	2.791
250 pg/ml	1.607	1.613	1.519
	1.619
125 pg/ml	0.871	0.882	0.788
	0.893
62.5 pg/ml	0.487	0.471	0.377
	0.454
31.25 pg/ml	0.303	0.284	0.19
	0.264
15.625 pg/ml	0.197	0.189	0.095
	0.181
7.8125 pg/ml	0.14	0.138	0.044
	0.136
0 pg/ml	0.093	0.094	0
	0.095

We measured random samples of BPE366 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	53.7	1.2	2.2
Intra-assay	2	20	158	4.2	2.6
Intra-assay	3	20	359	11	3.1
Inter-assay	1	20	53.8	3.4	6.4
Inter-assay	2	20	156	8	5.1
Inter-assay	3	20	361	22	6.1

> Protocol Booklet

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