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Home > Product > ELISA Primacu > Human Junctional Adhesion Molecule A, F11R Primacu™ ELISA Kit

Human Junctional Adhesion Molecule A, F11R Primacu™ ELISA Kit

Human Junctional Adhesion Molecule A, F11R Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.26 pg/mL

Detection range: 7.8-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.26 pg/mL

Detection range: 7.8-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Junctional adhesion molecule A

Code

BPE371

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.26 pg/mL

Detection range

7.8-500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

UniProt accession

MASS(Da)

32583

GeneID

50848

Synonyms

JAM1;JCAM

Gene names

F11R

Research area

Cardiovascular,Signal transduction

Target protein

JAM-A

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE371 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
500 pg/ml 2.551 2.497 2.394
2.443
250 pg/ml 1.37 1.396 1.293
1.422
125 pg/ml 0.767 0.796 0.693
0.825
62.5 pg/ml 0.441 0.45 0.347
0.458
31.25 pg/ml 0.295 0.286 0.183
0.277
15.625 pg/ml 0.197 0.202 0.099
0.207
7.8125 pg/ml 0.146 0.156 0.053
0.165
0 pg/ml 0.1 0.103 0
0.105

Precision

We measured random samples of BPE371 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 51.6 2.7 5.2
Intra-assay 2 20 105 3.9 3.7
Intra-assay 3 20 251 7.8 3.1
Inter-assay 1 20 51.7 4.5 8.8
Inter-assay 2 20 106 10.3 9.7
Inter-assay 3 20 249 24.9 10

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE371

    $2320.00

  • 96T

    BPE371

    $580.00

  • 15*96T

    BPE371

    $6525.00

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Lead time: Within two weeks

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