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Home > Product > ELISA Primacu > Human Slam Family Member 6, SLAMF6 Primacu™ ELISA Kit

Human Slam Family Member 6, SLAMF6 Primacu™ ELISA Kit

Human Slam Family Member 6, SLAMF6 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.2 pg/mL

Detection range: 7.03-450 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.2 pg/mL

Detection range: 7.03-450 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

SLAM family member 6

Code

BPE235

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.2 pg/mL

Detection range

7.03-450 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

SLAM family member 6, also known as Activating NK receptor, NK-T-B-antigen, NTB-A, SLAMF6, KALI and Ly18, is a single-pass type I membrane protein that belongs to the CD2 subfamily of the immunoglobulin superfamily. SLAMF6 / Ly18 contains one Ig-like (immunoglobulin-like) domain. It is expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. SLAMF6 / Ly18 triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. SLAMF6 / Ly18 is a homodimer. It interacts with PTN6 and, upon phosphorylation, with PTN11 and SH2D1A/SAP. SLAMF6 / Ly18 undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It may function as a coreceptor in the process of NK cell activation. SLAMF6 / Ly18 can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients.

UniProt accession

MASS(Da)

37345

GeneID

114836

Synonyms

KALI

Gene names

SLAMF6

Research area

Immunology

Target protein

SLAMF6

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE235 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
450 pg/ml 2.333 2.411 2.328
2.49
225 pg/ml 1.303 1.34 1.257
1.377
112.5 pg/ml 0.729 0.701 0.618
0.674
56.25 pg/ml 0.366 0.382 0.299
0.399
28.125 pg/ml 0.211 0.225 0.142
0.239
14.0625 pg/ml 0.152 0.156 0.073
0.16
7.03125 pg/ml 0.123 0.124 0.041
0.125
0 pg/ml 0.079 0.083 0
0.087

Precision

We measured random samples of BPE235 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 51 2.5 4.8
Intra-assay 2 20 170 7 4.1
Intra-assay 3 20 340 29.3 8.6
Inter-assay 1 20 51.1 3.3 6.4
Inter-assay 2 20 169 12.4 7.3
Inter-assay 3 20 344 23.7 6.9

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE235

    $2320.00

  • 96T

    BPE235

    $580.00

  • 15*96T

    BPE235

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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