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Home > Product > ELISA Primacu > Human Carboxypeptidase B, CPB1 Primacu™ ELISA Kit

Human Carboxypeptidase B, CPB1 Primacu™ ELISA Kit

Human Carboxypeptidase B, CPB1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.77 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.77 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carboxypeptidase B

Code

BPE096

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.77 pg/mL

Detection range

6.25-400 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

UniProt accession

MASS(Da)

47368

GeneID

1360

Synonyms

CPB;PCPB

Gene names

CPB1

Research area

Metabolism,Signal transduction

Target protein

Carboxypeptidase B1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE096 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400 pg/ml 2.877 2.971 2.872
3.064
200 pg/ml 1.526 1.463 1.364
1.399
100 pg/ml 0.836 0.783 0.684
0.73
50 pg/ml 0.408 0.433 0.334
0.457
25 pg/ml 0.273 0.274 0.175
0.275
12.5 pg/ml 0.171 0.184 0.085
0.196
6.25 pg/ml 0.142 0.135 0.036
0.127
0 pg/ml 0.097 0.099 0
0.1

Precision

We measured random samples of BPE096 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 17 1.2 6.8
Intra-assay 2 20 70.8 3.2 4.5
Intra-assay 3 20 208 7.9 3.8
Inter-assay 1 20 17.1 1.5 8.9
Inter-assay 2 20 71.4 3.8 5.3
Inter-assay 3 20 209 14.8 7.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE096

    $2320.00

  • 96T

    BPE096

    $580.00

  • 15*96T

    BPE096

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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