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Home > Product > ELISA Primacu > Human Interleukin-7, IL7 Primacu™ ELISA Kit

Human Interleukin-7, IL7 Primacu™ ELISA Kit

Human Interleukin-7, IL7 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.43 pg/mL

Detection range: 5.47-350 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.43 pg/mL

Detection range: 5.47-350 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-7

Code

BPE228

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.43 pg/mL

Detection range

5.47-350 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

20187

GeneID

3574

Gene names

IL7

Research area

Immunology,Neuroscience,Cancer

Target protein

IL-7

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE228 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
350 pg/ml 2.512 2.608 2.49
2.704
175 pg/ml 1.383 1.421 1.303
1.459
87.5 pg/ml 0.867 0.818 0.7
0.769
43.75 pg/ml 0.498 0.479 0.361
0.46
21.875 pg/ml 0.305 0.294 0.176
0.283
10.9375 pg/ml 0.209 0.2 0.082
0.19
5.46875 pg/ml 0.158 0.155 0.037
0.152
0 pg/ml 0.124 0.118 0
0.112

Precision

We measured random samples of BPE228 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 18.8 1 5.4
Intra-assay 2 20 58.7 1.5 2.5
Intra-assay 3 20 279 6.6 2.4
Inter-assay 1 20 18.8 1.9 10.2
Inter-assay 2 20 59.2 5.9 10
Inter-assay 3 20 282 25.9 9.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE228

    $2040.00

  • 96T

    BPE228

    $480.00

  • 15*96T

    BPE228

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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