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Home > Product > ELISA Primacu > Human Beta-Nerve Growth Factor, NGF Primacu™ ELISA Kit

Human Beta-Nerve Growth Factor, NGF Primacu™ ELISA Kit

Human Beta-Nerve Growth Factor, NGF Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.92 pg/mL

Detection range: 2.19-140 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.92 pg/mL

Detection range: 2.19-140 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Beta-nerve growth factor

Code

BPE167

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

0.92 pg/mL

Detection range

2.19-140 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

UniProt accession

MASS(Da)

26959

GeneID

4803

Synonyms

NGFB

Gene names

NGF

Research area

Neuroscience

Target protein

NGF

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE167 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
140 pg/ml 2.254 2.243 2.125
2.232
70 pg/ml 1.213 1.282 1.164
1.35
35 pg/ml 0.679 0.709 0.591
0.739
17.5 pg/ml 0.45 0.427 0.309
0.404
8.75 pg/ml 0.274 0.275 0.157
0.276
4.375 pg/ml 0.19 0.197 0.079
0.203
2.1875 pg/ml 0.165 0.158 0.04
0.151
0 pg/ml 0.123 0.118 0
0.113

Precision

We measured random samples of BPE167 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 9.5 0.65 6.8
Intra-assay 2 20 27.9 1.3 4.5
Intra-assay 3 20 111 4.4 4
Inter-assay 1 20 9.6 0.86 9
Inter-assay 2 20 27.8 1.5 5.3
Inter-assay 3 20 110 7.7 7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE167

    $2040.00

  • 96T

    BPE167

    $480.00

  • 15*96T

    BPE167

    $5400.00

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Lead time: Within two weeks

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