Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE124 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
125 pg/ml	2.634	2.528	2.433
	2.422
62.5 pg/ml	1.377	1.367	1.272
	1.356
31.25 pg/ml	0.755	0.759	0.664
	0.763
15.625 pg/ml	0.44	0.427	0.332
	0.414
7.8125 pg/ml	0.246	0.25	0.155
	0.254
3.90625 pg/ml	0.185	0.174	0.079
	0.162
1.953125 pg/ml	0.139	0.134	0.039
	0.129
0 pg/ml	0.093	0.095	0
	0.096

We measured random samples of BPE124 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	8.7	0.5	5.8
Intra-assay	2	20	32.4	1.3	4.1
Intra-assay	3	20	72	5.6	7.7
Inter-assay	1	20	8.7	0.73	8.4
Inter-assay	2	20	32.1	2.8	8.6
Inter-assay	3	20	71.6	7	9.7

> Protocol Booklet

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