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Home > Product > ELISA Kit > Human Semenogelin-1, SEMG1 ELISA Kit

Human Semenogelin-1, SEMG1 ELISA Kit

Human Semenogelin-1, SEMG1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Semenogelin-1

Code

E3266Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.16ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.

UniProt accession

MASS(Da)

52,131

GeneID

6406

Synonyms

Alpha-inhibin-31;Alpha-inhibin-92;Cancer/testis antigen 103;Semenogelin I;Semenogelin-1;SEMG 1;SEMG1;Seminal basic protein;SGI;

Gene names

SEMG1

Research area

Cell Biology

Target protein

SEMG1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3266Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.575 2.625 2.582
2.674
24ng/ml 1.588 1.576 1.533
1.564
12ng/ml 0.924 0.924 0.881
0.924
6ng/ml 0.427 0.44 0.397
0.453
3ng/ml 0.205 0.241 0.198
0.277
0ng/ml 0.052 0.043 0
0.034

Precision

We measured random samples of E3266Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.7 0.47 3.7
Intra-Assay 2 18 24.3 0.86 3.5
Intra-Assay 3 18 6.2 0.32 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E3266Hu

    $320.00

  • 96T

    E3266Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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