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Home > Product > ELISA Kit > Porcine Hydroxyacyl-coenzyme A Dehydrogenase, Mitochondrial, HADH ELISA Kit

Porcine Hydroxyacyl-coenzyme A Dehydrogenase, Mitochondrial, HADH ELISA Kit

Porcine Hydroxyacyl-coenzyme A Dehydrogenase, Mitochondrial, HADH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

Code

E0572Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.98ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed:9593854, PubMed:2817332). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity).

UniProt accession

MASS(Da)

34,161

GeneID

397604

Synonyms

HAD;HADH;HADHSC;HCDH;Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial;L-3-hydroxyacyl CoA dehydrogenase;Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase;Short-chain 3-hydroxyacyl-CoA dehydrogenase

Gene names

HADH

Research area

Signal Transduction; Cancer; Metabolism

Target protein

HADH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0572Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 1.788 1.831 1.777
1.875
1200ng/L 1.09 1.088 1.034
1.087
600ng/L 0.415 0.458 0.404
0.5
300ng/L 0.281 0.27 0.216
0.259
150ng/L 0.126 0.122 0.068
0.117
0ng/L 0.051 0.054 0
0.057

Precision

We measured random samples of E0572Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1562.74 49.56 3.17
Intra-Assay 2 18 553.33 23.84 4.31
Intra-Assay 3 18 1401.54 66.96 4.78

Protocols

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  • Size

    Code

    Price

  • 48T

    E0572Po

    $320.00

  • 96T

    E0572Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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