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Home > Product > ELISA Kit > Porcine Insulin-like Growth Factor 1 Receptor, IGF1R ELISA Kit

Porcine Insulin-like Growth Factor 1 Receptor, IGF1R ELISA Kit

Porcine Insulin-like Growth Factor 1 Receptor, IGF1R ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Insulin-like growth factor 1 receptor

Code

E0574Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.76ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (By similarity). When present in a hybrid receptor with INSR, binds IGF1 (By similarity).

UniProt accession

MASS(Da)

34,615

GeneID

397350

Synonyms

CD antigen CD221;Fragments;IGF1R;IGF-I receptor;Insulin-like growth factor 1 receptor;Insulin-like growth factor I receptor

Gene names

IGF1R

Research area

Cardiovascular; Signal Transduction; Cancer; Developmental Biology

Target protein

IGF1R

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0574Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.136 2.082 2.043
2.028
1200ng/L 1.569 1.514 1.475
1.459
600ng/L 0.62 0.62 0.581
0.62
300ng/L 0.338 0.385 0.346
0.432
150ng/L 0.178 0.19 0.151
0.202
0ng/L 0.021 0.039 0
0.057

Precision

We measured random samples of E0574Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 721.85 27.35 3.79
Intra-Assay 2 18 1680.07 77.02 4.58
Intra-Assay 3 18 554.49 26.31 4.74

Protocols

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  • Size

    Code

    Price

  • 48T

    E0574Po

    $320.00

  • 96T

    E0574Po

    $458.00

Add to Cart

Lead time: Within two weeks

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