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Home > Product > ELISA Kit > Porcine Mucin-1, MUC1 ELISA Kit

Porcine Mucin-1, MUC1 ELISA Kit

Porcine Mucin-1, MUC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 34.55U/mL

Detection range: 70-15000U/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 34.55U/mL

Detection range: 70-15000U/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Mucin-1

Code

E0587Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

34.55U/mL

Detection range

70-15000U/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Mucin 1, cell surface associated (MUC1), also called polymorphic epithelial mucin (PEM) or epithelial membrane antigen or EMA, is a mucin encoded by the MUC1 gene in humans. MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins protect the body from infection by pathogen binding to oligosaccharides in the extracellular domain, preventing the pathogen from reaching the cell surface. Overexpression of MUC1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. Joyce Taylor-Papadimitriou identified and characterised the antigen during her work with breast and ovarian tumors.

UniProt accession

MASS(Da)

223,173

GeneID

100125538

Synonyms

MUC1;MyHC-2x;Myosin heavy chain 1;Myosin heavy chain 2x;Myosin heavy chain, skeletal muscle, adult 1;Myosin-1

Gene names

MYH1

Research area

Tags & Cell Markers; Signal Transduction; Cancer

Target protein

MUC1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0587Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000U/mL 2.112 2.164 2.103
2.216
4000U/mL 1.476 1.501 1.44
1.525
2000U/mL 0.854 0.875 0.815
0.897
1000U/mL 0.502 0.471 0.411
0.441
500U/mL 0.301 0.272 0.211
0.242
0U/mL 0.053 0.061 0
0.068

Precision

We measured random samples of E0587Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5774.07 256.05 4.43
Intra-Assay 2 18 1044.63 63.57 6.09
Intra-Assay 3 18 1964.66 92.34 4.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0587Po

    $320.00

  • 96T

    E0587Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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