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Home > Product > ELISA Kit > Porcine Pulmonary Surfactant-associated Protein B, SP-B ELISA Kit

Porcine Pulmonary Surfactant-associated Protein B, SP-B ELISA Kit

Porcine Pulmonary Surfactant-associated Protein B, SP-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.32ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 3.32ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Pulmonary surfactant-associated protein B

Code

E0599Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.32ng/mL

Detection range

7-1500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

UniProt accession

MASS(Da)

8,714

GeneID

397296

Synonyms

8 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTP3;SP-B

Gene names

SFTPB

Research area

Immunology; Microbiology; Signal Transduction; Cell Biology; Cancer

Target protein

SP-B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0599Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/mL 2.345 2.191 2.147
2.037
400ng/mL 1.716 1.712 1.668
1.708
200ng/mL 1.198 1.229 1.185
1.26
100ng/mL 0.659 0.663 0.619
0.667
50ng/mL 0.436 0.419 0.375
0.402
0ng/mL 0.053 0.044 0
0.035

Precision

We measured random samples of E0599Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 77.33 3.95 5.11
Intra-Assay 2 18 216.28 12.79 5.91
Intra-Assay 3 18 248.47 11.62 4.68

Protocols

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  • Size

    Code

    Price

  • 48T

    E0599Po

    $320.00

  • 96T

    E0599Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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