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Home > Product > ELISA Kit > Porcine Toll-like Receptor 4, TLR4 ELISA Kit

Porcine Toll-like Receptor 4, TLR4 ELISA Kit

Porcine Toll-like Receptor 4, TLR4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Toll-like receptor 4

Code

E0608Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.58ng/mL

Detection range

1-400ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (By similarity).

UniProt accession

MASS(Da)

96,309

GeneID

399541

Synonyms

CD antigen CD284;TLR4;Toll-like receptor 4

Gene names

TLR4

Research area

Immunology; Microbiology; Cardiovascular

Target protein

TLR4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0608Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/mL 2.235 2.288 2.225
2.341
120ng/mL 1.377 1.416 1.354
1.455
60ng/mL 0.695 0.682 0.619
0.668
30ng/mL 0.426 0.492 0.429
0.557
15ng/mL 0.255 0.248 0.186
0.242
0ng/mL 0.068 0.063 0
0.057

Precision

We measured random samples of E0608Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 39.79 1.9 4.78
Intra-Assay 2 18 66.78 2.81 4.2
Intra-Assay 3 18 112.97 3.89 3.44

Protocols

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  • Size

    Code

    Price

  • 48T

    E0608Po

    $320.00

  • 96T

    E0608Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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