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Home > Product > ELISA Kit > Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.21ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 2.21ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Code

E0611Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.21ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (By similarity). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).

UniProt accession

MASS(Da)

24,859

GeneID

396637

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1;Ubiquitin thioesterase L1;UCHL1;UCH-L1

Gene names

UCHL1

Research area

Neuroscience; Cell Biology; Tags & Cell Markers

Target protein

UCHL1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0611Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.037 2.131 2.089
2.226
320ng/L 1.801 1.774 1.731
1.746
160ng/L 1.276 1.232 1.19
1.188
80ng/L 0.622 0.618 0.575
0.613
40ng/L 0.398 0.402 0.36
0.406
0ng/L 0.032 0.042 0
0.053

Precision

We measured random samples of E0611Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 114.64 6.6 5.76
Intra-Assay 2 18 186.32 6.61 3.55
Intra-Assay 3 18 49.66 2.95 5.94

Protocols

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  • Size

    Code

    Price

  • 48T

    E0611Po

    $320.00

  • 96T

    E0611Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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