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Home > Product > ELISA Kit > Rat Fibronectin, FN ELISA Kit

Rat Fibronectin, FN ELISA Kit

Rat Fibronectin, FN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Fibronectin

Code

E0633Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

UniProt accession

MASS(Da)

272,511

GeneID

25661

Synonyms

Anastellin];FIBNEC;Fibronectin;FN;fn-1

Gene names

Fn1

Research area

Cardiovascular; Signal Transduction; Stem Cells; Cancer; Developmental Biology

Target protein

FN

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0633Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.168 2.222 2.173
2.277
12ng/ml 1.945 1.841 1.792
1.737
6ng/ml 1.214 1.183 1.134
1.153
3ng/ml 0.652 0.653 0.604
0.654
1.5ng/ml 0.383 0.382 0.333
0.381
0ng/ml 0.055 0.049 0
0.043

Precision

We measured random samples of E0633Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.88 0.28 5.76
Intra-Assay 2 18 8.31 0.36 4.33
Intra-Assay 3 18 2.89 0.18 6.28

Protocols

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  • Size

    Code

    Price

  • 48T

    E0633Ra

    $320.00

  • 96T

    E0633Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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