Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Guanine Nucleotide Exchange Factor Vav3, VAV3 ELISA Kit

Human Guanine Nucleotide Exchange Factor Vav3, VAV3 ELISA Kit

Human Guanine Nucleotide Exchange Factor Vav3, VAV3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Guanine nucleotide exchange factor VAV3

Code

E0645Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion (By similarity).

UniProt accession

MASS(Da)

97,776

GeneID

10451

Synonyms

Guanine nucleotide exchange factor VAV3;VAV3;VAV-3

Gene names

VAV3

Research area

Signal Transduction; Cancer

Target protein

VAV3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0645Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.183 2.199 2.171
2.216
6ng/ml 1.126 1.148 1.119
1.169
3ng/ml 0.833 0.821 0.792
0.809
1.5ng/ml 0.493 0.5 0.471
0.507
0.75ng/ml 0.24 0.225 0.196
0.21
0ng/ml 0.03 0.029 0
0.028

Precision

We measured random samples of E0645Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.58 0.1 6.55
Intra-Assay 2 18 6.06 0.2 3.24
Intra-Assay 3 18 4.39 0.3 6.81

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E0645Hu

    $320.00

  • 96T

    E0645Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up