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Home > Product > ELISA Kit > Human Tnf Receptor-associated Factor 2, TRAF2 ELISA Kit

Human Tnf Receptor-associated Factor 2, TRAF2 ELISA Kit

Human Tnf Receptor-associated Factor 2, TRAF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.28ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.28ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tnf Receptor-Associated Factor 2

Code

E3268Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.28ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.

UniProt accession

MASS(Da)

55,859

GeneID

7186

Synonyms

E3 ubiquitin-protein ligase TRAF2;RING-type E3 ubiquitin transferase TRAF2;TNF receptor-associated factor 2;TRAF 2;TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3

Gene names

TRAF2

Research area

Cell Biology

Target protein

TRAF2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3268Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.224 2.113 2.078
2.002
1600ng/L 1.313 1.409 1.374
1.505
800ng/L 0.609 0.585 0.55
0.56
400ng/L 0.398 0.371 0.336
0.344
200ng/L 0.124 0.133 0.099
0.143
0ng/L 0.044 0.035 0
0.025

Precision

We measured random samples of E3268Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1585.8 52.57 3.3
Intra-Assay 2 18 1052.2 43.87 4.2
Intra-Assay 3 18 635.3 27.4 4.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3268Hu

    $320.00

  • 96T

    E3268Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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