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Home > Product > ELISA Kit > Human Signal Transducer and Activator of Transcription 2, STAT2 ELISA Kit

Human Signal Transducer and Activator of Transcription 2, STAT2 ELISA Kit

Human Signal Transducer and Activator of Transcription 2, STAT2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

signal transducer and activator of transcription 2

Code

E0675Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed:9020188, PubMed:23391734). In addition, has also a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs (PubMed:28165510). Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed:26122121, PubMed:23391734, PubMed:9020188).

UniProt accession

MASS(Da)

97,916

GeneID

6773

Synonyms

IMD44;ISGF-3;P113;PTORCH3;Signal transducer and activator of transcription 2;STAT113;STAT2

Gene names

STAT2

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Microbiology

Target protein

STAT2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0675Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.016 2.151 2.102
2.286
3.2ng/ml 1.474 1.431 1.382
1.389
1.6ng/ml 0.951 0.852 0.803
0.753
0.8ng/ml 0.531 0.521 0.472
0.512
0.4ng/ml 0.3 0.263 0.214
0.227
0ng/ml 0.044 0.049 0
0.055

Precision

We measured random samples of E0675Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.18 0.12 5.54
Intra-Assay 2 18 3.92 0.19 4.8
Intra-Assay 3 18 0.7 0.03 4.76

Protocols

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  • Size

    Code

    Price

  • 48T

    E0675Hu

    $320.00

  • 96T

    E0675Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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