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Home > Product > ELISA Kit > Human Vinculin, VCL ELISA Kit

Human Vinculin, VCL ELISA Kit

Human Vinculin, VCL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

vinculin

Code

E0684Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.32ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

UniProt accession

MASS(Da)

123,799

GeneID

7414

Synonyms

CMD1W;CMH15;HEL114;Metavinculin;MV;MVCL;VCL ;Vinculin

Gene names

VCL

Research area

Signal Transduction; Cardiovascular

Target protein

VCL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0684Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.338 2.363 2.302
2.387
1000ng/L 1.421 1.409 1.348
1.397
500ng/L 0.679 0.736 0.675
0.792
250ng/L 0.451 0.463 0.403
0.476
125ng/L 0.24 0.272 0.212
0.305
0ng/L 0.07 0.061 0
0.052

Precision

We measured random samples of E0684Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1151.01 27.74 2.41
Intra-Assay 2 18 276.46 17.25 6.24
Intra-Assay 3 18 736.82 34.09 4.63

Protocols

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  • Size

    Code

    Price

  • 48T

    E0684Hu

    $320.00

  • 96T

    E0684Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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