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Home > Product > ELISA Kit > Human Glyceraldehyde-3-phosphate Dehydrogenase, GAPDH ELISA Kit

Human Glyceraldehyde-3-phosphate Dehydrogenase, GAPDH ELISA Kit

Human Glyceraldehyde-3-phosphate Dehydrogenase, GAPDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

glyceraldehyde-3-phosphate dehydrogenase

Code

E0704Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) (EC 1.2.1.12) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed

UniProt accession

MASS(Da)

36,053

GeneID

2597

Synonyms

G3PD;GAPD;GAPDH;Glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;Peptidyl-cysteine S-nitrosylase GAPDH

Gene names

GAPDH

Research area

Neuroscience; Signal Transduction; Cancer; Metabolism

Target protein

GAPDH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0704Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.124 2.1 2.059
2.077
12ng/ml 1.145 1.18 1.139
1.216
6ng/ml 0.693 0.729 0.688
0.765
3ng/ml 0.508 0.461 0.419
0.413
1.5ng/ml 0.208 0.223 0.182
0.237
0ng/ml 0.056 0.041 0
0.026

Precision

We measured random samples of E0704Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.02 0.54 5.35
Intra-Assay 2 18 18.25 0.57 3.12
Intra-Assay 3 18 4.43 0.27 6.18

Protocols

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  • Size

    Code

    Price

  • 48T

    E0704Hu

    $320.00

  • 96T

    E0704Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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