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Home > Product > ELISA Kit > Mouse Bone Morphogenetic Protein 5, BMP5 ELISA Kit

Mouse Bone Morphogenetic Protein 5, BMP5 ELISA Kit

Mouse Bone Morphogenetic Protein 5, BMP5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Bone Morphogenetic Protein 5

Code

E0773Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.58ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis (PubMed:7958439, PubMed:29321139). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation (By similarity).

UniProt accession

MASS(Da)

51,512

GeneID

12160

Synonyms

AU023399;BMP5;BMP-5;Bone morphogenetic protein 5;se

Gene names

Bmp5

Research area

Cardiovascular; Signal Transduction; Cell Biology

Target protein

BMP5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0773Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.001 2.006 1.947
2.012
120ng/L 1.032 1.012 0.953
0.993
60ng/L 0.467 0.516 0.457
0.565
30ng/L 0.264 0.239 0.18
0.214
15ng/L 0.09 0.11 0.051
0.13
0ng/L 0.059 0.059 0
0.059

Precision

We measured random samples of E0773Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 196.86 5.6 2.84
Intra-Assay 2 18 78.35 3.36 4.29
Intra-Assay 3 18 137.73 5.03 3.65

Protocols

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  • Size

    Code

    Price

  • 48T

    E0773Mo

    $320.00

  • 96T

    E0773Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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