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Home > Product > ELISA Kit > Human Matrix Metalloproteinase 9, MMP-9 ELISA Kit

Human Matrix Metalloproteinase 9, MMP-9 ELISA Kit

Human Matrix Metalloproteinase 9, MMP-9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 15.12ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 15.12ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Matrix Metalloproteinase 9/Gelatinase B

Code

E0936Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

15.12ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.

UniProt accession

MASS(Da)

78,458

GeneID

4318

Synonyms

67 kDa Matrix Metalloproteinase-9;82 kDa Matrix Metalloproteinase-9];92 kDa gelatinase;92 kDa type IV collagenase;CLG4B;Gelatinase B;GELB;MANDP2;Matrix Metalloproteinase-9;MMP-9

Gene names

MMP9

Research area

Cancer; Cell Biology

Target protein

MMP-9

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0936Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.117 2.236 2.179
2.355
2400ng/L 1.285 1.416 1.359
1.547
1200ng/L 0.727 0.771 0.714
0.814
600ng/L 0.54 0.505 0.448
0.471
300ng/L 0.22 0.243 0.186
0.267
0ng/L 0.042 0.057 0
0.071

Precision

We measured random samples of E0936Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1563.3 101.33 6.48
Intra-Assay 2 18 3312.33 110.21 3.33
Intra-Assay 3 18 497.72 25.3 5.08

Protocols

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  • Size

    Code

    Price

  • 48T

    E0936Hu

    $320.00

  • 96T

    E0936Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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