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Home > Product > ELISA Kit > Rat Nadphoxidase 4, NOX4 ELISA Kit

Rat Nadphoxidase 4, NOX4 ELISA Kit

Rat Nadphoxidase 4, NOX4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

NADPH oxidase 4

Code

E1298Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.

UniProt accession

MASS(Da)

66,469

GeneID

85431

Synonyms

Kidney oxidase-1;Kidney superoxide-producing NADPH oxidase;KOX-1;NADPH oxidase 4;NOX4

Gene names

Nox4

Research area

Cardiovascular; Signal Transduction; Metabolism; Cell Biology

Target protein

NOX4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1298Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 1.795 1.899 1.852
2.003
16ng/ml 1.112 1.113 1.065
1.113
8ng/ml 0.627 0.593 0.546
0.558
4ng/ml 0.186 0.202 0.155
0.219
2ng/ml 0.079 0.092 0.045
0.105
0ng/ml 0.055 0.047 0
0.039

Precision

We measured random samples of E1298Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.02 0.39 4.93
Intra-Assay 2 18 19.25 0.54 2.8
Intra-Assay 3 18 15.73 0.87 5.53

Protocols

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  • Size

    Code

    Price

  • 48T

    E1298Ra

    $320.00

  • 96T

    E1298Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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