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Home > Product > ELISA Kit > Human Estradiol 17-beta-dehydrogenase 8, HSD17B8 ELISA Kit

Human Estradiol 17-beta-dehydrogenase 8, HSD17B8 ELISA Kit

Human Estradiol 17-beta-dehydrogenase 8, HSD17B8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.09ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.09ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Estradiol 17-Beta-Dehydrogenase 8

Code

E3327Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.09ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (PubMed: 17978863). The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed: 19571038, PubMed: 25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed: 25203508).

UniProt accession

MASS(Da)

26,974

GeneID

7923

Synonyms

17-beta-HSD 8;17-beta-hydroxysteroid dehydrogenase 8;3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit;3-oxoacyl-[acyl-carrier-protein] reductase;Estradiol 17-beta-dehydrogenase 8;HSD17B8;KAR alpha subunit;Ke-6;Protein Ke6;Really interesting new gene 2 protein;Short chain dehydrogenase/reductase family 30C member 1;Testosterone 17-beta-dehydrogenase 8

Gene names

HSD17B8

Research area

Signal Transduction

Target protein

HSD17B8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3327Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.147 2.281 2.221
2.414
1200ng/L 1.39 1.295 1.235
1.2
600ng/L 0.853 0.875 0.815
0.897
300ng/L 0.477 0.458 0.398
0.438
150ng/L 0.324 0.296 0.236
0.267
0ng/L 0.063 0.06 0
0.057

Precision

We measured random samples of E3327Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1034.4 58.37 5.6
Intra-Assay 2 18 1690.8 54.43 3.2
Intra-Assay 3 18 488.5 37.54 7.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E3327Hu

    $320.00

  • 96T

    E3327Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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