Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat 3 Beta-Hydroxysteroid Dehydrogenase, Delta 5-->4-isomerase Type 1, HSD3B1 ELISA Kit

Rat 3 Beta-Hydroxysteroid Dehydrogenase, Delta 5-->4-isomerase Type 1, HSD3B1 ELISA Kit

Rat 3 Beta-Hydroxysteroid Dehydrogenase, Delta 5-->4-isomerase Type 1, HSD3B1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Code

E1432Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone (PubMed:1537836, PubMed:1985917). Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1537836). Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).

UniProt accession

MASS(Da)

42,037

GeneID

360348

Synonyms

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I;3-beta-HSD I;3-beta-hydroxy-5-ene steroid dehydrogenase;3-beta-hydroxy-Delta(5-steroid dehydrogenase;3-beta-hydroxysteroid 3-dehydrogenase;Delta-5-3-ketosteroid isomerase;Dihydrotestosterone oxidoreductase;HSD3B1;Steroid Delta-isomerase

Gene names

Hsd3b1

Research area

Signal Transduction; Cancer; Metabolism

Target protein

HSD3B1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1432Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.26 2.209 2.161
2.158
6ng/ml 1.616 1.656 1.609
1.697
3ng/ml 0.965 1.048 1
1.131
1.5ng/ml 0.774 0.765 0.717
0.757
0.75ng/ml 0.404 0.442 0.394
0.479
0ng/ml 0.047 0.048 0
0.049

Precision

We measured random samples of E1432Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.02 0.06 6.26
Intra-Assay 2 18 4.39 0.2 4.47
Intra-Assay 3 18 3.64 0.27 7.38

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E1432Ra

    $320.00

  • 96T

    E1432Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up