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Home > Product > ELISA Kit > Rat Steroid 21-hydroxylase, CYP21A2 ELISA Kit

Rat Steroid 21-hydroxylase, CYP21A2 ELISA Kit

Rat Steroid 21-hydroxylase, CYP21A2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.31ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 10.31ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Steroid 21-hydroxylase

Code

E1500Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.31ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

UniProt accession

MASS(Da)

55,946

GeneID

24298

Synonyms

21-OH;21-OHase;Cyp21;CYP21A2;Cytochrome P450 21;Cytochrome P450 XXI;Cytochrome P450-C21;Cytochrome P-450c21;Steroid 21-hydroxylase;

Gene names

Cyp21

Research area

Cardiovascular

Target protein

CYP21A2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1500Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.342 2.342 2.309
2.343
1600ng/L 1.354 1.443 1.409
1.532
800ng/L 0.815 0.821 0.788
0.828
400ng/L 0.424 0.442 0.408
0.46
200ng/L 0.294 0.257 0.223
0.22
0ng/L 0.033 0.034 0
0.034

Precision

We measured random samples of E1500Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1530.18 52.24 3.41
Intra-Assay 2 18 285.14 13.29 4.66
Intra-Assay 3 18 697.45 32.92 4.72

Protocols

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  • Size

    Code

    Price

  • 48T

    E1500Ra

    $320.00

  • 96T

    E1500Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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