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Home > Product > ELISA Kit > Rat Lanosterol 14 Alpha Demethylase, CYP51A1 ELISA Kit

Rat Lanosterol 14 Alpha Demethylase, CYP51A1 ELISA Kit

Rat Lanosterol 14 Alpha Demethylase, CYP51A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Lanosterol 14 alpha demethylase

Code

E1518Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid (PubMed:7665087, PubMed:10544287, PubMed:11328599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7665087, PubMed:10544287, PubMed:11328599).

UniProt accession

MASS(Da)

56,707

GeneID

25427

Synonyms

Cyp51a1;CYPLI;Cytochrome P450 51A1;Cytochrome P45014DM;Cytochrome P450-14DM;Cytochrome P450LI;Lanosterol 14-alpha demethylase;LDM;RATCP14DM;Sterol 14-alpha demethylase

Gene names

Cyp51a1

Research area

Cardiovascular

Target protein

Cyp51a1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1518Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.242 2.174 2.142
2.105
6ng/ml 1.323 1.389 1.357
1.455
3ng/ml 0.761 0.768 0.736
0.775
1.5ng/ml 0.378 0.381 0.349
0.383
0.75ng/ml 0.168 0.153 0.122
0.139
0ng/ml 0.025 0.032 0
0.038

Precision

We measured random samples of E1518Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.98 0.25 5.05
Intra-Assay 2 18 8.5 0.33 3.91
Intra-Assay 3 18 2.13 0.1 4.61

Protocols

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  • Size

    Code

    Price

  • 48T

    E1518Ra

    $320.00

  • 96T

    E1518Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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