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Home > Product > ELISA Kit > Mouse Proto-oncogene Wnt-1, WNT1 ELISA Kit

Mouse Proto-oncogene Wnt-1, WNT1 ELISA Kit

Mouse Proto-oncogene Wnt-1, WNT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.24ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.24ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Proto-oncogene Wnt-1

Code

E1566Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.24ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (By similarity). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (PubMed:15454084, PubMed:16116452). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (PubMed:2202907, PubMed:16116452). Has a role in osteoblast function, bone development and bone homeostasis (By similarity).

UniProt accession

MASS(Da)

41,086

GeneID

22408

Synonyms

Int;Int-1;Proto-oncogene Int-1;Proto-oncogene Wnt-1;sw;swaying;Wnt-;WNT1;Wnt-1;

Gene names

Wnt1

Research area

Stem Cells

Target protein

WNT1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1566Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.15 2.176 2.129
2.203
600ng/L 1.382 1.398 1.351
1.414
300ng/L 0.831 0.886 0.839
0.942
150ng/L 0.535 0.48 0.433
0.425
75ng/L 0.248 0.232 0.185
0.217
0ng/L 0.036 0.047 0
0.058

Precision

We measured random samples of E1566Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 217 13.96 6.43
Intra-Assay 2 18 880.53 40.77 4.63
Intra-Assay 3 18 278.38 16.21 5.82

Protocols

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  • Size

    Code

    Price

  • 48T

    E1566Mo

    $320.00

  • 96T

    E1566Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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