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Home > Product > ELISA Kit > Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12, ADAMTS12 ELISA Kit

Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12, ADAMTS12 ELISA Kit

Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 12, ADAMTS12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.32ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.32ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Adisintegrin and metalloproteinase with thrombospondin motifs 12

Code

E1724Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.32ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cleaves aggrecan, a cartilage proteoglycan, at the '1683-Glu-|-Leu-1684' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).

UniProt accession

MASS(Da)

105,706

GeneID

79252

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 1;ADAM-TS 1;ADAMTS-1;ADAM-TS1;ADAMTS12

Gene names

Adamts1

Research area

Signal Transduction; Cancer

Target protein

ADAMTS12

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1724Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 1.837 1.833 1.784
1.83
32ng/ml 1.139 1.081 1.032
1.023
16ng/ml 0.624 0.568 0.519
0.511
8ng/ml 0.252 0.27 0.221
0.288
4ng/ml 0.133 0.11 0.061
0.087
0ng/ml 0.057 0.049 0
0.041

Precision

We measured random samples of E1724Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.69 0.84 5.33
Intra-Assay 2 18 29.87 1.53 5.12
Intra-Assay 3 18 55.56 2.32 4.18

Protocols

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  • Size

    Code

    Price

  • 48T

    E1724Ra

    $320.00

  • 96T

    E1724Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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