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Home > Product > ELISA Kit > Mouse Acrosin-binding Protein, ACRBP ELISA Kit

Mouse Acrosin-binding Protein, ACRBP ELISA Kit

Mouse Acrosin-binding Protein, ACRBP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Acrosin-binding protein

Code

E1811Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acrosomal protein that maintains proacrosin (pro-ACR) as an enzymatically inactive zymogen in the acrosome. Involved also in the acrosome formation. Maintains pro-ACR as an enzymatically inactive zymogen in the acrosome until acrosomal exocytosis (PubMed:27303034). Partially contributes also to the assembly of acrosomal proteins to form an acrosomal granule (PubMed:27303034). Rodent specific isoform that participates in the formation of the acrosomal granule into the center of the acrosomal vesicle during early spermiogenesis (PubMed:27303034). In the fertilization process promotes ACR release from the acrosome during acrosomal exocytosis (PubMed:27303034).

UniProt accession

MASS(Da)

61,141

GeneID

54137

Synonyms

ACRBP;Acrosin-binding protein;Acrosin-binding protein, 32 kDa form, mature form;Acrosin-binding protein, 60 kDa form;Acrosin-binding protein, mature form;ACRPB-C;OY-TES;OY-TES-1;Proacrosin-binding protein sp32;sp;sp32;Sp32]

Gene names

Acrbp

Research area

Tags & Cell Markers; Cancer

Target protein

ACRBP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1811Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 1.836 1.813 1.765
1.789
6ng/mL 1.084 1.021 0.974
0.959
3ng/mL 0.616 0.62 0.573
0.625
1.5ng/mL 0.221 0.257 0.21
0.293
0.75ng/mL 0.14 0.114 0.066
0.087
0ng/mL 0.035 0.047 0
0.06

Precision

We measured random samples of E1811Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.12 0.29 4.78
Intra-Assay 2 18 8.56 0.3 3.55
Intra-Assay 3 18 2.14 0.1 4.78

Protocols

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  • Size

    Code

    Price

  • 48T

    E1811Mo

    $320.00

  • 96T

    E1811Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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