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Home > Product > ELISA Kit > Human Bh3 Interacting Domain Death Agonist, BID ELISA Kit

Human Bh3 Interacting Domain Death Agonist, BID ELISA Kit

Human Bh3 Interacting Domain Death Agonist, BID ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

BH3 interacting domain death agonist

Code

E1831Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

UniProt accession

MASS(Da)

21,995

GeneID

637

Synonyms

BH3-interacting domain death agonist;BH3-interacting domain death agonist p11;BH3-interacting domain death agonist p13;BH3-interacting domain death agonist p15;Bid;FP497;p11 BID];p13 BID;p15 BID;p22 BID

Gene names

BID

Research area

Cell Biology

Target protein

Bid

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1831Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.554 2.473 2.428
2.393
6ng/ml 1.475 1.501 1.456
1.528
3ng/ml 0.904 0.925 0.879
0.946
1.5ng/ml 0.518 0.523 0.477
0.529
0.75ng/ml 0.232 0.213 0.167
0.194
0ng/ml 0.028 0.046 0
0.063

Precision

We measured random samples of E1831Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.85 0.21 3.56
Intra-Assay 2 18 1.31 0.09 6.8
Intra-Assay 3 18 2.57 0.14 5.34

Protocols

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  • Size

    Code

    Price

  • 48T

    E1831Hu

    $320.00

  • 96T

    E1831Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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