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Home > Product > ELISA Kit > Human Apoptosis Protease Activating Factor-1, APAF-1 ELISA Kit

Human Apoptosis Protease Activating Factor-1, APAF-1 ELISA Kit

Human Apoptosis Protease Activating Factor-1, APAF-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.14ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.14ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

apoptosis protease activating factor-1

Code

E1835Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.14ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

UniProt accession

MASS(Da)

141,840

GeneID

317

Synonyms

APAF-1;Apoptotic protease-activating factor 1;CED4

Gene names

APAF1

Research area

Cell Biology

Target protein

Apaf-1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1835Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.393 2.278 2.233
2.163
800ng/L 1.377 1.406 1.36
1.434
400ng/L 0.625 0.652 0.607
0.679
200ng/L 0.333 0.335 0.29
0.337
100ng/L 0.135 0.17 0.125
0.205
0ng/L 0.042 0.045 0
0.049

Precision

We measured random samples of E1835Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 228.97 7.52 3.28
Intra-Assay 2 18 581.47 24.18 4.16
Intra-Assay 3 18 774.99 19.51 2.52

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1835Hu

    $320.00

  • 96T

    E1835Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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