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Home > Product > ELISA Kit > Human Receptor Tyrosine-protein Kinase Erbb-2, ERBB2 ELISA Kit

Human Receptor Tyrosine-protein Kinase Erbb-2, ERBB2 ELISA Kit

Human Receptor Tyrosine-protein Kinase Erbb-2, ERBB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Receptor Tyrosine-Protein Kinase Erbb-2

Code

E3376Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-38ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

UniProt accession

MASS(Da)

137,910

GeneID

2064

Synonyms

CD antigen CD340;ERBB 2;ERBB2;Metastatic lymph node gene 19 protein;MLN 19;p185erbB2;Proto-oncogene c-ErbB-2;Proto-oncogene Neu;Receptor tyrosine-protein kinase erbB-2;Tyrosine kinase-type cell surface receptor HER2

Gene names

ERBB2

Research area

Tags & Cell Markers; Signal Transduction; Cancer

Target protein

ERBB2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3376Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.064 2.127 2.063
2.19
10ng/ml 1.317 1.315 1.251
1.314
5ng/ml 0.852 0.874 0.809
0.895
2.5ng/ml 0.531 0.493 0.429
0.456
1.25ng/ml 0.282 0.255 0.191
0.228
0ng/ml 0.072 0.064 0
0.057

Precision

We measured random samples of E3376Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.2 0.15 7.1
Intra-Assay 2 18 5.1 0.31 6
Intra-Assay 3 18 10.7 0.41 3.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3376Hu

    $320.00

  • 96T

    E3376Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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