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Home > Product > ELISA Kit > Human Phosphatidylinositol Binding Clathrin Assembly Protein, PICALM ELISA Kit

Human Phosphatidylinositol Binding Clathrin Assembly Protein, PICALM ELISA Kit

Human Phosphatidylinositol Binding Clathrin Assembly Protein, PICALM ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Phosphatidylinositol Binding Clathrin Assembly Protein

Code

E3382Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.092ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed: 10436022, PubMed: 16262731, PubMed: 27574975). Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed: 25898166). In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed: 22118466, PubMed: 21808019, PubMed: 23741335). In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed: 25241929). Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF) (PubMed: 25241929, PubMed: 24067654).

UniProt accession

MASS(Da)

70,755

GeneID

8301

Synonyms

Clathrin assembly lymphoid myeloid leukemia protein;Phosphatidylinositol-binding clathrin assembly protein;PICALM

Gene names

PICALM

Research area

Others

Target protein

PICALM

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3382Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.181 2.137 2.097
2.092
20ng/ml 1.341 1.267 1.227
1.193
10ng/ml 0.715 0.787 0.747
0.859
5ng/ml 0.512 0.504 0.464
0.497
2.5ng/ml 0.265 0.242 0.202
0.219
0ng/ml 0.037 0.04 0
0.042

Precision

We measured random samples of E3382Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5 0.32 6.5
Intra-Assay 2 18 17.7 0.93 5.3
Intra-Assay 3 18 29.5 0.83 2.8

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3382Hu

    $320.00

  • 96T

    E3382Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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