Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Alpha-Methylacyl-Coa Recemase

Code

E3387Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.16ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359).

UniProt accession

MASS(Da)

42,387

GeneID

23600

Synonyms

2-methylacyl-CoA racemase;Alpha-methylacyl-CoA racemase;AMACR

Gene names

AMACR

Research area

Others

Target protein

AMACR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3387Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.436 2.436 2.389
2.436
1200ng/L 1.433 1.412 1.364
1.39
600ng/L 0.944 0.928 0.881
0.912
300ng/L 0.487 0.464 0.417
0.442
150ng/L 0.255 0.251 0.204
0.248
0ng/L 0.042 0.047 0
0.052

Precision

We measured random samples of E3387Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 545.4 30.62 5.6
Intra-Assay 2 18 1506.3 52.96 3.5
Intra-Assay 3 18 496.3 31.3 6.3

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E3387Hu

    $320.00

  • 96T

    E3387Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up