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Home > Product > ELISA Kit > Human Cytochrome P450 1B1, CYP1B1 ELISA Kit

Human Cytochrome P450 1B1, CYP1B1 ELISA Kit

Human Cytochrome P450 1B1, CYP1B1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.235ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.235ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 1B1

Code

E3398Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.235ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 20972997, PubMed: 11555828, PubMed: 12865317, PubMed: 10681376, PubMed: 15258110). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 20972997, PubMed: 11555828, PubMed: 12865317, PubMed: 10681376, PubMed: 15258110). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position (PubMed: 11555828, PubMed: 12865317). Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (PubMed: 10426814). May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376, PubMed: 15258110). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed: 20972997). Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (PubMed: 21068195). Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (PubMed: 10426814). Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (By similarity).

UniProt accession

MASS(Da)

60,846

GeneID

1545

Synonyms

CYP1B1;CYPIB1;Cytochrome P450 1B1;Hydroperoxy icosatetraenoate dehydratase

Gene names

CYP1B1

Research area

Others

Target protein

CYP1B1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3398Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
96ng/ml 1.815 1.876 1.844
1.938
48ng/ml 0.913 1.01 0.977
1.106
24ng/ml 0.447 0.424 0.391
0.401
12ng/ml 0.18 0.231 0.198
0.282
6ng/ml 0.085 0.121 0.089
0.157
0ng/ml 0.033 0.033 0
0.033

Precision

We measured random samples of E3398Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 27.1 0.89 3.3
Intra-Assay 2 18 84.7 3.24 3.8
Intra-Assay 3 18 46.8 2.3 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E3398Hu

    $320.00

  • 96T

    E3398Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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