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Home > Product > ELISA Kit > Mouse Eosinophil Peroxidase, EPO ELISA Kit

Mouse Eosinophil Peroxidase, EPO ELISA Kit

Mouse Eosinophil Peroxidase, EPO ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Eosinophil Peroxidase

Code

E2055Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Eosinophil peroxidase is an enzyme found within the eosinophil granulocytes, innate immune cells of humans and mammals. This oxidoreductase protein is encoded by the gene EPX, expressed within these myeloid cells. EPO shares many similarities with its orthologous peroxidases, myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). The protein is concentrated in secretory granules within eosinophils. Eosinophil peroxidase is a heme peroxidase, its activities including the oxidation of halide ions to bacteriocidal reactive oxygen species, the cationic disruption of bacterial cell walls, and the post-translational modification of protein amino acid residues.

UniProt accession

MASS(Da)

81,380

GeneID

13861

Synonyms

Eosinophil peroxidase;Eosinophil peroxidase heavy chain];Eosinophil peroxidase light chain;EPO

Gene names

Epx

Research area

Immunology

Target protein

EPO

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2055Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.208 2.163 2.125
2.119
6ng/mL 1.516 1.472 1.434
1.429
3ng/mL 0.769 0.764 0.726
0.759
1.5ng/mL 0.441 0.412 0.374
0.383
0.75ng/mL 0.213 0.176 0.138
0.139
0ng/mL 0.048 0.038 0
0.028

Precision

We measured random samples of E2055Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.61 0.22 3.34
Intra-Assay 2 18 2.81 0.12 4.1
Intra-Assay 3 18 1.59 0.07 4.32

Protocols

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  • Size

    Code

    Price

  • 48T

    E2055Mo

    $320.00

  • 96T

    E2055Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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