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Home > Product > ELISA Kit > Human Beta-1, 3-N-acetylglucosaminyltransferase Radical Fringe, RFNG ELISA Kit

Human Beta-1, 3-N-acetylglucosaminyltransferase Radical Fringe, RFNG ELISA Kit

Human Beta-1, 3-N-acetylglucosaminyltransferase Radical Fringe, RFNG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.14ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.14ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Beta-1,3-N-acetylglucosaminyltransferase radical fringe

Code

E2100Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.14ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1. May be involved in limb formation and in neurogenesis.

UniProt accession

MASS(Da)

36,424

GeneID

5986

Synonyms

Beta-1,3-N-acetylglucosaminyltransferase radical fringe;O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase;RFNG

Gene names

RFNG

Research area

Immunology

Target protein

RFNG

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2100Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.442 2.316 2.248
2.19
1200ng/L 1.392 1.316 1.248
1.239
600ng/L 0.897 0.847 0.779
0.797
300ng/L 0.419 0.413 0.345
0.408
150ng/L 0.254 0.273 0.205
0.291
0ng/L 0.064 0.068 0
0.072

Precision

We measured random samples of E2100Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1049.73 52.7 5.02
Intra-Assay 2 18 1677.6 56.06 3.34
Intra-Assay 3 18 273.37 18.01 6.59

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2100Hu

    $320.00

  • 96T

    E2100Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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