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Home > Product > ELISA Kit > Human Aldo-keto Reductase Family 1, Member B10, AKR1B10 ELISA Kit

Human Aldo-keto Reductase Family 1, Member B10, AKR1B10 ELISA Kit

Human Aldo-keto Reductase Family 1, Member B10, AKR1B10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aldo-Keto Reductase Family 1, Member B10

Code

E3401Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed: 9565553, PubMed: 18087047, PubMed: 12732097, PubMed: 19013440, PubMed: 19563777). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed: 12732097, PubMed: 18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed: 19013440, PubMed: 19563777). Displays no reductase activity towards glucose (PubMed: 12732097).

UniProt accession

MASS(Da)

36,020

GeneID

57016

Synonyms

AKR1B1;AKR1B10;Aldo-keto reductase family 1 member B10;Aldose reductase-like;Aldose reductase-related protein;ARL-1;ARP;hARP;SI reductase;Small intestine reductase

Gene names

AKR1B10

Research area

Metabolism

Target protein

AKR1B10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.048 2.105 2.055
2.163
3.2ng/ml 1.643 1.647 1.597
1.651
1.6ng/ml 1.023 1.015 0.965
1.007
0.8ng/ml 0.729 0.754 0.704
0.778
0.4ng/ml 0.411 0.422 0.372
0.433
0ng/ml 0.06 0.05 0
0.04

Precision

We measured random samples of E3401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.1 0.07 6.2
Intra-Assay 2 18 3.5 0.2 5.8
Intra-Assay 3 18 0.9 0.07 7.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E3401Hu

    $320.00

  • 96T

    E3401Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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