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Home > Product > ELISA Kit > Mouse Interferon Regulatory Factor 5, IRF5 ELISA Kit

Mouse Interferon Regulatory Factor 5, IRF5 ELISA Kit

Mouse Interferon Regulatory Factor 5, IRF5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.54ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.54ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

interferon regulatory factor 5

Code

E2163Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.54ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:15665823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:15665823). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (PubMed:15665823).

UniProt accession

MASS(Da)

56,005

GeneID

27056

Synonyms

AW491843;Interferon regulatory factor 5;IRF 5;IRF5;IRF-5;mir;mirf5

Gene names

Irf5

Research area

Immunology; Epigenetics and Nuclear Signaling

Target protein

IRF5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2163Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.16 2.199 2.146
2.237
240ng/L 1.272 1.275 1.223
1.279
120ng/L 0.678 0.791 0.738
0.904
60ng/L 0.455 0.473 0.421
0.492
30ng/L 0.312 0.285 0.232
0.259
0ng/L 0.047 0.053 0
0.058

Precision

We measured random samples of E2163Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 210.41 9.38 4.46
Intra-Assay 2 18 380.24 15.76 4.14
Intra-Assay 3 18 66.06 4.94 7.48

Protocols

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  • Size

    Code

    Price

  • 48T

    E2163Mo

    $320.00

  • 96T

    E2163Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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