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Home > Product > ELISA Kit > Rat Occludin, OCLN ELISA Kit

Rat Occludin, OCLN ELISA Kit

Rat Occludin, OCLN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.37ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.37ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Occludin

Code

E2185Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.37ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.

UniProt accession

MASS(Da)

59,186

GeneID

83497

Synonyms

Occludin;Ocln

Gene names

Ocln

Research area

Signal Transduction

Target protein

Ocln

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2185Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.139 2.107 2.072
2.075
800ng/L 1.393 1.356 1.322
1.319
400ng/L 0.739 0.775 0.741
0.812
200ng/L 0.517 0.468 0.433
0.419
100ng/L 0.245 0.248 0.213
0.25
0ng/L 0.039 0.035 0
0.03

Precision

We measured random samples of E2185Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1006.02 40.22 4
Intra-Assay 2 18 389.5 21.15 5.43
Intra-Assay 3 18 273.98 14.53 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2185Ra

    $320.00

  • 96T

    E2185Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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