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Home > Product > ELISA Kit > Bovine Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Bovine Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Bovine Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.016ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.016ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Peroxisome Proliferation Activated Receptor Gamma

Code

E2258Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.016ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.

UniProt accession

MASS(Da)

57,579

GeneID

281993

Synonyms

Nuclear receptor subfamily 1 group C member 3;Peroxisome proliferator-activated receptor gamma;PPAR G;PPAR gamma;PPARG;PPAR-gamma

Gene names

PPARG

Research area

Others

Target protein

PPARG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2258Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.062 2.174 2.129
2.285
6ng/ml 1.492 1.475 1.43
1.458
3ng/ml 0.896 0.913 0.868
0.929
1.5ng/ml 0.434 0.491 0.447
0.549
0.75ng/ml 0.238 0.265 0.22
0.292
0ng/ml 0.035 0.045 0
0.054

Precision

We measured random samples of E2258Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.7 0.29 4.3
Intra-Assay 2 18 4.6 0.3 6.5
Intra-Assay 3 18 1.7 0.09 5.3

Protocols

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  • Size

    Code

    Price

  • 96T

    E2258Bo

    $458.00

  • 48T

    E2258Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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