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Home > Product > ELISA Kit > Bovine C-C Motif Chemokine 8, CCL8 ELISA Kit

Bovine C-C Motif Chemokine 8, CCL8 ELISA Kit

Bovine C-C Motif Chemokine 8, CCL8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.19ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 7.19ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

C-C motif chemokine 8

Code

E2198Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.19ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Chemokine (C-C motif) ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B, CCR3 and CCR5.

UniProt accession

MASS(Da)

10,900

GeneID

281044

Synonyms

C-C motif chemokine 8;CCL8;MCP-2;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;SCYA8;Small-inducible cytokine A8

Gene names

CCL8

Research area

Immunology

Target protein

CCL8

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2198Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.274 2.198 2.134
2.122
800ng/L 1.203 1.253 1.189
1.303
400ng/L 0.74 0.745 0.681
0.751
200ng/L 0.543 0.518 0.454
0.494
100ng/L 0.258 0.248 0.184
0.239
0ng/L 0.078 0.064 0
0.05

Precision

We measured random samples of E2198Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 179.65 14.12 7.86
Intra-Assay 2 18 1051.65 33.9 3.22
Intra-Assay 3 18 488.58 27.28 5.58

Protocols

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  • Size

    Code

    Price

  • 48T

    E2198Bo

    $320.00

  • 96T

    E2198Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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