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Home > Product > ELISA Kit > Bovine Decorin, DCN ELISA Kit

Bovine Decorin, DCN ELISA Kit

Bovine Decorin, DCN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.31ng/mL

Detection range: 0.6-180ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 0.31ng/mL

Detection range: 0.6-180ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Decorin

Code

E2204Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.31ng/mL

Detection range

0.6-180ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Decorin is a protein that in humans is encoded by the DCN gene. Decorin is a proteoglycan that is on average 90 - 140 kilodaltons (kDa) in molecular weight. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly.

UniProt accession

MASS(Da)

39,879

GeneID

280760

Synonyms

Bone proteoglycan II;DCN;Decorin;PG-S2

Gene names

DCN

Research area

Others

Target protein

DCN

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2204Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
96ng/mL 2.136 2.004 1.953
1.872
48ng/mL 1.169 1.066 1.016
0.964
24ng/mL 0.593 0.573 0.523
0.553
12ng/mL 0.284 0.294 0.244
0.305
6ng/mL 0.095 0.13 0.079
0.165
0ng/mL 0.037 0.051 0
0.065

Precision

We measured random samples of E2204Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 42.75 1.54 3.6
Intra-Assay 2 18 82.39 2.41 2.92
Intra-Assay 3 18 30.52 1.44 4.71

Protocols

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  • Size

    Code

    Price

  • 48T

    E2204Bo

    $320.00

  • 96T

    E2204Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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