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Home > Product > ELISA Kit > Human Transcription Factor 7, TCF7 ELISA Kit

Human Transcription Factor 7, TCF7 ELISA Kit

Human Transcription Factor 7, TCF7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Transcription Factor 7

Code

E3430Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.054ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

UniProt accession

MASS(Da)

41,642

GeneID

6932

Synonyms

T-cell factor 1;T-cell-specific transcription factor 1;TCF 7;TCF-1;TCF7;TCF-7;Transcription factor 7

Gene names

TCF7

Research area

Epigenetics and Nuclear Signaling

Target protein

TCF7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3430Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.282 2.327 2.288
2.373
12ng/ml 1.325 1.297 1.257
1.269
6ng/ml 0.702 0.684 0.644
0.666
3ng/ml 0.466 0.468 0.428
0.471
1.5ng/ml 0.202 0.193 0.153
0.184
0ng/ml 0.053 0.04 0
0.027

Precision

We measured random samples of E3430Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.8 0.39 3.1
Intra-Assay 2 18 5.5 0.32 5.7
Intra-Assay 3 18 11.1 0.73 6.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E3430Hu

    $320.00

  • 96T

    E3430Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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