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Home > Product > ELISA Kit > Bovine Thioredoxin Reductase 1, TXNRD1 ELISA Kit

Bovine Thioredoxin Reductase 1, TXNRD1 ELISA Kit

Bovine Thioredoxin Reductase 1, TXNRD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.057mU/mL

Detection range: 0.1-40mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 0.057mU/mL

Detection range: 0.1-40mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Thioredoxin reductase 1

Code

E2252Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.057mU/mL

Detection range

0.1-40mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Thioredoxin reductase 1, cytoplasmic is an enzyme that in humans is encoded by the TXNRD1 gene. This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.

UniProt accession

MASS(Da)

54,770

GeneID

282388

Synonyms

Thioredoxin reductase 1, cytoplasmic;Thioredoxin reductase TR1;TR;TXNRD1

Gene names

TXNRD1

Research area

Signal Transduction

Target protein

TXNRD1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2252Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24mU/mL 2.075 2.164 2.112
2.254
12mU/mL 1.24 1.314 1.261
1.388
6mU/mL 0.65 0.673 0.62
0.696
3mU/mL 0.379 0.398 0.345
0.417
1.5mU/mL 0.173 0.212 0.159
0.251
0mU/mL 0.056 0.053 0
0.05

Precision

We measured random samples of E2252Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.3 0.45 3.67
Intra-Assay 2 18 6.54 0.31 4.71
Intra-Assay 3 18 3.42 0.21 6.16

Protocols

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  • Size

    Code

    Price

  • 48T

    E2252Bo

    $320.00

  • 96T

    E2252Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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