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Home > Product > ELISA Kit > Rat Steroid 17-alpha-hydroxylase, CYP17A1 ELISA Kit

Rat Steroid 17-alpha-hydroxylase, CYP17A1 ELISA Kit

Rat Steroid 17-alpha-hydroxylase, CYP17A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Steroid 17-alpha-hydroxylase/17,20 lyase

Code

E2264Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.75ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

UniProt accession

MASS(Da)

57,250

GeneID

25146

Synonyms

17-alpha-hydroxyprogesterone aldolase;Cyp17;CYP17A1;CYPXVII;Cytochrome P450 17A1;Cytochrome P450c17;Cytochrome P450-C17;Steroid 17-alpha-hydroxylase/17,20 lyase;Steroid 17-alpha-monooxygenase;

Gene names

Cyp17a1

Research area

Cardiovascular

Target protein

CYP17A1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2264Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.484 2.428 2.381
2.371
800ng/L 1.562 1.489 1.443
1.417
400ng/L 0.82 0.848 0.802
0.877
200ng/L 0.494 0.482 0.436
0.471
100ng/L 0.275 0.248 0.201
0.221
0ng/L 0.064 0.047 0
0.029

Precision

We measured random samples of E2264Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 241.29 14.71 6.1
Intra-Assay 2 18 797.44 29.2 3.66
Intra-Assay 3 18 372.91 18.41 4.94

Protocols

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  • Size

    Code

    Price

  • 48T

    E2264Ra

    $320.00

  • 96T

    E2264Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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