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Home > Product > ELISA Kit > Rat Transferrin Receptor Protein 1, CD71 ELISA Kit

Rat Transferrin Receptor Protein 1, CD71 ELISA Kit

Rat Transferrin Receptor Protein 1, CD71 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.08ng/mL

Detection range: 10-2000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.08ng/mL

Detection range: 10-2000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Transferrin receptor protein 1/CD antigen CD71

Code

E2286Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.08ng/mL

Detection range

10-2000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (By similarity). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Positively regulates T and B cell proliferation through iron uptake (By similarity). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (By similarity). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (By similarity). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (By similarity).

UniProt accession

MASS(Da)

85,876

GeneID

64678

Synonyms

CD antigen CD71;TFR;TfR1;TR;Transferrin receptor protein 1;Trfr

Gene names

Tfrc

Research area

Cardiovascular; Immunology

Target protein

TFR

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2286Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/mL 2.189 2.271 2.219
2.354
600ng/mL 1.248 1.25 1.198
1.253
300ng/mL 0.622 0.699 0.647
0.777
150ng/mL 0.354 0.388 0.336
0.422
75ng/mL 0.214 0.193 0.14
0.171
0ng/mL 0.052 0.052 0
0.053

Precision

We measured random samples of E2286Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 295.12 11.9 4.03
Intra-Assay 2 18 513.38 23.82 4.64
Intra-Assay 3 18 626.9 19.03 3.04

Protocols

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  • Size

    Code

    Price

  • 48T

    E2286Ra

    $320.00

  • 96T

    E2286Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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